Evaluating the effect of ethanol foliar feeding on the essential oil, phenolic content, and antioxidant activities of Ducrosia anethifolia

Volume 5, Issue 04, Pages 66-76, Dec 2022 *** Field: Analytical Environmental Chemistry

  • Aliyeh Sarabandi Department of Phytochemistry, Faculty of Science, University of Sistan and Baluchistan, Zahedan, Iran
  • Amirhossein Sahebkar Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. and Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  • Javad Asili Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  • Moharam Valizadeh Faculty of Environmental Sciences and Sustainable Agriculture, Sistan and Baluchistan University, Zahedan, Iran
  • Khalilollah Taheri Department of Biology, Faculty of Science, University of Sistan and Baluchistan, Zahedan, Iran
  • Jafar Valizadeh, Corresponding Author, Department of Biology, Faculty of Science, University of Sistan and Baluchistan, Zahedan, Iran
  • Maryam Akaberi, Corresponding Author, Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Keywords: Ducrosia anethifolia, GC-MS, Spectrophotometry, Foliar feeding, Essential oil, Antioxidant


Ducrosia anethifolia (Apiaceae) is a medicinal aromatic plant distributed in Iran and Afghanistan. This research aims to investigate the composition of the plant essential oil, determine the total flavonoid and phenolic contents, and evaluate its antioxidant activities after ethanol foliar feeding. For this purpose, 0, 10, 20, 40, and 80% v/v of aqueous ethanol solutions were sprayed on different batches of the plants. Then, the essential oils were obtained using water distillation. Compounds were analyzed by Gas chromatography-mass spectrometry technique(GC-MS) using a validated method. The method was validated as per the ICH guidelines for linearity, precision, accuracy, robustness, LOD, and LOQ. The total contents of phenols and flavonoids were measured using spectrophotometric methods. The antioxidant activity was evaluated using DPPH and FRAP assays. The results showed that n-decanal, cis-verbenyl acetate and dodecanal were the major compounds in all treatments. However, alcohol could cause significant differences in the essential oils qualitatively and quantitatively. The results showed that 40% ethanol could increase the number of phenolics and flavonoids and consequently the antioxidant activity.  Thus, ethanol foliar feeding can be used as an appropriate approach to increase the essential oil of D. anethifolia as well as its phenolic and flavonoid contents.


J. Mottaghipisheh, A. Boveiri Dehsheikh, M. Mahmoodi Sourestani, T. Kiss, J. Hohmann, D. Csupor, Ducrosia spp., rare plants with promising phytochemical and pharmacological characteristics: An updated review, Pharmaceuticals (Basel), 13 (2020) 175. https://doi.org/10.3390/ph13080175

M. Arbabi, H. Naghdi Badi, M. Labbafi, A. Mehrafarin, E. Saboki, Investigating the essential oil composition of Ducrosia anethifolia (DC.) Boiss. in different altitudes of Sistan and Baluchestan province, Iran, J. Med. Plants, 19 (2020) 343-355. https://doi.org/10.29252/jmp.19.74.343

J. Mottaghipisheh, M.T. Maghsoudlou, J. Valizadeh, R. Arjomandi, Antioxidant activity and chemical composition of the essential oil of Ducrosia anethifolia (DC.) Boiss. from Neyriz, J. Med. Plant. By-prod., 2 (2014) 215-218. https://doi.org/10.22092/JMPB.2014.108737.

E.R. Elsharkawy, E.M. Abdallah, M.H. Shiboob, S.M. Alghanem, Phytochemical, antioxidant and antibacterial potential of Ducrosia anethifolia in northern border region of Saudi Arabia, J. Pharm. Res. Int., 31 (2019) 1-8. https://doi.org/10.9734/jpri/2019/v31i630361

N.M. Shalaby, H.I. Abd-Alla, H.F. Aly, M.A. Albalawy, K.H. Shaker, J. Bouajila, Preliminary in vitro and in vivo evaluation of antidiabetic activity of Ducrosia anethifolia Boiss. and its linear furanocoumarins, Biomed. Res. Int., 2014 (2014) 480545. https://doi.org/10.1155/2014/480545

M. Zamyad, M. Abasnejad, S. Esmaeili-Mahani, A. Mostafavi, Alpha-Pinene as the main component of Ducrosia anethifolia (Boiss) essential oil is responsible for its effect on locomotor activity in rats, Avicenna J. Neuro. Psycho. Physiol., 3 (2016) 29-34. ‎ https://doi.org/10.17795/ajnpp-38787.

M. Akaberi, Z. Tayarani-Najaran, I. Mehregan, J. Asili, A. Sahebkar, M. Hassanzadeh-Khayyat, S.A. Emami, Review of the essential oil composition of Iranian endemic and native taxa of Apiaceae (Umbelliferae), Curr. Org. Chem., 24 (2020) 909-1009. https://doi.org/10.2174/1385272824999200513103632.

M. Abbasnejad, A. Mostafavi, R. Kooshki, P. Hamzenejad, S. Esmaeili-Mahani, Effect of Ducrosia anethifolia (Dc.) Bioss essential oil on spatial learning and memory in rats, J. Gorgan Univ. Med. Sci., 18 (2017) 9-15. http://goums.ac.ir/journal/browse.php?a_id=2949&sid=1&slc_lang=en

L. Obeidi, A.A. Mehrabi, M. Omidi, A. Oladzad, Meiotic behavior and pollen viability of Ducrosia anethifolia (DC), Iran. J. Rangelands For. Plant Breed. Genet. Res., 20 (2012) 124-133. https://doi.org 10.22092/IJRFPBGR.2012.6631

M. Zamyad, M. Abbasnejad, S. Esmaeili-Mahani, A. Mostafavi, V. Sheibani, The anticonvulsant effects of Ducrosia anethifolia (Boiss) essential oil are produced by its main component alpha-pinene in rats, Arq. Neuropsiquiatr., 77 (2019) 106-114. https://doi.org/10.1590/0004-282X20180147.

M. Karami, F. Ghassemi, Effects of aqueous-alcoholic extract of Ducrosia anethifolia (DC.) Boiss. leaves on fetal heart tissue in diabetic rats, Iran. J. Med. Aromatic Plants Res., 37 (2021) 52-64. https://doi.org/10.22092/IJMAPR.2021.342950.2791

N. Rahimi, E. Samani Jahromi, S. Zolghadri Jahromi, The effect of the hydro-alcoholic extract of (Ducrosia anethifolia) on testosterone hormone and the histological changes of the testicle in male adult rats, Armaghane-danesh, Yasuj Univ. Med. Sci. J., 21 (2016) 682-693. https://armaghanj.yums.ac.ir/article-1-1389-en.pdf

L. Shooshtari, M. Omidi, E. Majidi, M. R. Naghavi, M. Ghorbanpour, A. Etminan, Assessment of somaclonal variation of regenerated Ducrosia anethifolia plants using AFLP markers, Journal of Horticulture, Forestry Biotechnol., 17 (2013) 99-106. https://journal-hfb.usab-tm.ro/romana/2013/Lista%20Lucrari%20PDF/Lucrari%20Vol%2017(4)%20PDF/22Lia%20Shooshtari.pdf

C. Wang, L. Guo, Y. Li, Z. Wang, Systematic comparison of C3 and C4 plants based on metabolic network analysis, BMC Syst. Biol., 6 (2012) S9. https://doi.org/10.1186/1752-0509-6-S2-S9

S. Sajedi Moghadam, A. Mehrafarin, H. Naghdi Badi, A.R. Pazoki, N. Qavami, Evaluation of phytochemical yield of thyme (Thymus vulgaris L.) under foliar application of hydroalcohols, J. Med. Plants, 4 (2012) 130-140. https://doi.org/20.1001.1.2717204.2012.

J. Asili, Z. Tayarani-Najaran, S.A. Emami, M. Iranshahi, A. Sahebkar, S. Eghbali, Chemical composition, cytotoxic and antibacterial activity of essential oil from aerial parts of Salvia tebesana Bunge., J. Essen. Oil-Bear. Plants, 24 (2021) 31-39. https://doi.org/10.1080/0972060X.2021.1886996

Z. Sadeghi, J. Valizadeh, O. Azyzian Shermeh, M. Akaberi, Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchestan, Iran, Avicenna J. Phytomed., 5 (2015) 1-9. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352527/

E.V. Beketov, V. P. Pakhomov, O.V. Nesterova, Improved method of flavonoid extraction from bird cherry fruits, Pharm. Chem. J., 39 (2005) 316-318. https://doi.org/10.1007/s11094-005-0143-7

M. Jahani, M. Akaberi, M. Hasanzadeh Khayyat, S.A. Emami, Chemical composition and antioxidant activity of essential oils from Cupressus sempervirens. var. sempervirens, C. sempervirens cv. Cereiformis and C. sempervirens var. horizentalis, J. Essent. Oil-Bear. Plants, 22 (2019) 917-931. https://doi.org/10.1080/0972060X.2019.1646672

H. Nourafcan, Z. Kalantari, The effect of methanol and ethanol foliar application on peppermint morpho-physiological charactristics, Agroecol. J., 12 (2017) 1-9. http://www.Agroecology Journal.com

I. Zbiec, C.O. Podsiad, Response of some cultivated plants to methanol as compared to supplemental irrigation, Electron. J. Pol. Agric. Univ., 6 (2003) 1-7. http://www.ejpau.media.pl

M.I. Makhdum, M.N.A. Malik, S. Din, F. Ahmad, F.I. Chaudhry, Physiological response of cotton to methanol foliar application, J. Res. Sci., 13 (2002) 37-43. https://www.bzu.edu.pk/jrscience/vol13no1/5.pdf

M.N. Safarazade Vishgahi, G. Nourmohamadi, H. Magidi, H. Effect of methanol on peanut function and yield components, Iran. J. Agric. Sci. 103 (2007) 88. https://jijas.ut.ac.ir

H. Hadadi, P. Moradi, F. Matlabi, The Effect of methanol and manganese salt spraying on the amount and components of essential oil of melissa (Melissa officinalis L.), J. Med. Plants, 2 (2016) 80-88. https://doi.org/20.1001.1.2717204.2016.

V. Hajhashemi, M. Rabbani, A. Ghanadi, E. Davari, Evaluation of antianxiety and sedative effects of essential oil of Ducrosia anethifolia in mice, Clinics, 21 (1985) 5103-5121. https://doi.org/10.1590/S1807-59322010001000020

F. Sefidkon, I. Javidtash, Essential oil composition of Ducrosia anethifolia (DC.) Boiss. from Iran, J. Essential Oil Res., 14 (2002) 278-279. https://doi.org/20.1001.1.2717204.2020.

S. Salari, M. Shamsaddini, The production of nanoliposomal system containing Ducrosia anethifolia (DC.) Boiss essential oil made by sonication and filtration methods, J. Med. Plants, 19 (2020) 229-238. https://doi.org/20.1001.1.2717204.2020.

Z. Karimi, A. Ghani, S. Mohtashami, Comparative study of essential oil content and composition of Ducrosia anethifolia at two phenologocal stage, Res. J. Pharm., 44 (2017) 29-30. http://rjpharmacognosy.ir

M. Keshavarzi, A. Sharifan, S.A. Yasini Ardakani, Effect of the essential oil of Ducrosia anethifolia (DC.) Boiss. and Teucrium polium L. on physicochemical, sensory, and microbial characteristics of probiotic yogurt during storage time, Iran. J. Food Sci. Technol., 18 (2021) 265-276. https://doi.org/10.52547/fsct.18.117.265

How to Cite
Sarabandi, A., Sahebkar, A., Asili, J., Valizadeh, M., Taheri, K., Valizadeh, J., & Akaberi, M. (2022). Evaluating the effect of ethanol foliar feeding on the essential oil, phenolic content, and antioxidant activities of Ducrosia anethifolia. Analytical Methods in Environmental Chemistry Journal, 5(04), 66-76. https://doi.org/10.24200/amecj.v5.i04.213
Original Article