Chemical analysis of Eucalyptus and Rosemary essential oils using gas chromatography-mass spectrometry (GC-MS) and evaluation of their toxicity against the most common urban pest

Volume 6, Issue 03, Pages 89-102, Sep 2023 *** Field: Analytical method in plant

  • Marzieh Asgari MSc student of Biology and Vector Control, School of Public Health, Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Moloud Kazemi Department of Pharmaceutics, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Mohammad Mahmoudi Sourastani Department of Horticultural Sciences, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
  • Mona Sharififard, Corresponding Author, Department of Medical Entomology and Vector Control, School of Public Health, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Keywords: Gas chromatography-mass spectrometry, Eucalyptus, Rosemary, Essential oil, Urban pest, Toxicity


Using plant essential oils (EOs) as a pesticide alternative has gained increasing interest as a promising strategy to reduce the harmful effects of chemical pesticides. This study aims to investigate the chemical composition of Eucalyptus globulus Labill and Rosmarinus officinalis L. essential oils and evaluate their impacts against Blattella germanica L. under laboratory conditions. The essential oils were prepared from dried leaves using hydro-distillation (HD) as a chemical extraction method. The gas chromatography-mass spectrometry (GC-MS) was employed to analyze and identify their chemical compounds. Bioassays were conducted using the standard contact method recommended by the World Health Organization, and the data were analyzed using the probit regression model. By GC-MS analysis, the major components included 1,8-cineole (50.67%), alpha-pinene (17.48%), limonene (4.26%) for eucalyptus and alpha-pinene (20.67%), camphor (10.69%), 1,8-cineole (9.38%), Borneol (9.02%), comphene (7.15%), and limonene (4.88%) for rosemary. The LD50 values were 9. 27, 10.54, and 3.23 %, and LD95 values increased to 27.2, 22.3 %, and 14.3% for rosemary, eucalyptus, and their mixture. The EOs mixture had a higher repellent effect with a repellency rate of 98.9% at a concentration of 3% compared to 93.3% and 90% at a concentration of 5% for rosemary and eucalyptus alone. 


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How to Cite
Asgari, M., Kazemi, M., Mahmoudi Sourastani, M., & Sharififard, M. (2023). Chemical analysis of Eucalyptus and Rosemary essential oils using gas chromatography-mass spectrometry (GC-MS) and evaluation of their toxicity against the most common urban pest. Analytical Methods in Environmental Chemistry Journal, 6(03), 89-102.
Original Article