Spectrofluorometric determination of L-tryptophan after preconcentration using multi-walled carbon nanotubes
Vol 2, Issue 01, Pages 43-48 **** Filed: Bioanalysis
Abstract
A solid-phase extraction method based on multi-walled carbon nanotubes (MWCNTs) was applied for the preconcentration of L-tryptophan prior to its spectrofluorometric determination. Due to the high surface area of MWCNTs, satisfactory concentration factor and extraction recovery can be achieved with only 10 mg MWCNTs in 5 min. The effects of pH, sorbent amount, eluent type and time on the recovery of the analyte were investigated. Under the optimum conditions, the detection limit for L-tryptophan was 8.9 ng mL−1. The precision of the method, evaluated as the relative standard deviation obtained by analyzing of 10 replicates, was 2.6%. The practical applicability of the developed method was examined using wheat and barley samples.
References
W. Amelung, X. Zhang, K.W. Flach, Amino acids in grassland soils: climatic effects on concentrations and chirality, Geoderma, 130 (2006) 207-217.
M. Ikeda, Amino Acid Production Processes. Adv. Biochem. Eng. Biotechnol. 79 (2002) 1-35.
C. Delgado-Andrade, J.A. Rufián-Hanares, S. Jiménez-Pérez, F.J. Morales, Tryptophan determination in milk-based ingredients and dried sport supplements by liquid chromatography with fluorescence detection, Food Chem., 98 (2006) 580-585.
D.I. Sanchez-Machado, B. Chavira-Willys, J. Lopez-Cervantes, High-performance liquid chromatography with fluorescence detection for quantitation of tryptophan and tyrosine in a shrimp waste protein concentrate, J Chromatogr., 863 (2008) 88–93.
Takagai Y, Igarashi S. Determination of ppb levels of tryptophan derivatives by capillary electrophoresis with homogeneous liquid–liquid extraction and sweeping method, Chem. Pharm. Bull., 51 (2003) 373-377.
A.R. Fiorucci, E.T.G. Cavalheiro, The use of carbon paste electrode in the direct voltammetric determination of tryptophan in pharmaceutical formulations, J. Pharm. Biomed. Anal., 28 (2002) 909-915.
J. Ren, M. Zhao, J. Wang, C. Cui, B. Yang, Spectrophotometric method for determination of tryptophan in protein hydrolysates, Food Technol. Biotechnol., 45 (2007) 360-366.
A.M. Othmana, S. Lib, R.M. Leblancb, Enhancing selectivity in spectrofluorimetric determination of tryptophan by using graphene oxide nanosheets, Anal. Chim. Acta, 787 (2013) 226-232.
H. Abdolmohammad-Zadeh, S.M. Hammami-Oskooyi, Solid-phase extraction of l-tryptophan from food samples utilizing a layered double hydroxide nano-sorbent prior to its determination by spectrofluorometry, J. Iran. Chem. SOC., 12 (2015) 1115-1122.
X. Zhu, Y. Gu, Modified nano-TiO2 coupled with fluorescence spectroscopy for the separation/analysis of L-tryptophan, Anal. Biochem., 401 (2010) 260-265.
E. Zolfonoun, A. Rouhollahi, A. Semnani, Solid-phase extraction and determination of ultra trace amounts of lead, mercury and cadmium in water samples using octadecyl silica membrane disks modified with 5,50-dithiobis(2-nitrobenzoic acid) and atomic absorption spectrometry, Int. J. Environ. Anal. Chem., 88 (2008) 327-336.
N. Wanga, X. Liang, Q. Li, Y. Liao, S. Shao, Nitro-substituted 3,3′-bis(indolyl)methane-modified silica gel as a sorbent for solid-phase extraction of flavonoids, RSC Adv., 5 (2015) 15500-15506.
E. Yavuz, S. Tokalıoğlu, H. Erkılıç, C. Soykan, Novel chelating resin for solid-phase extraction of metals in certified reference materials and waters, Anal. Lett., 50 (2017) 364-378.
J.B. Ghasemi, E. Zolfonoun, Simultaneous spectrophotometric determination of trace amounts of uranium, thorium, and zirconium using the partial least squares method after their preconcentration by α-benzoin oxime modified Amberlite XAD-2000 resin, Talanta, 80 (2010) 1191-1197.
K. Saberyan, E. Zolfonoun, M. Shamsipur, M. Salavati-Niasari, Separation and preconcentration of trace gallium and indium by amberlite XAD-7 resin impregnated with a new hexadentates naphthol-derivative schiff base, Sep. Sci. Technol., 44 (2009) 1851-1868.
Z.A. Alothman, E. Yilmaz, M. Habila, M. Soylak, Solid phase extraction of metal ions in environmental samples on 1-(2-pyridylazo)-2-naphthol impregnated activated carbon cloth, Ecotoxicol. Environ. Saf., 112 (2015) 74-79.
H. Ebrahimzadeh, M. Behbahani, A novel lead imprinted polymer as the selective solid phase for extraction and trace detection of lead ions by flame atomic absorption spectrophotometry: Synthesis, characterization and analytical application, Arab. J. Chem., 12 (2017) 2499-2508.
R.H. Baughman, A.A. Zakhidov, W.A. deHeer, Carbon nanotubes-the route toward applications, Science, 297 (2002) 787-792.
D. Pardasani, P.K. Kanaujia, A.K. Purohit, A.R. Shrivastava, D.K. Dubey, Magnetic multi-walled carbon nanotubes assisted dispersive solid phase extraction of nerve agents and their markers from muddy water, Talanta, 86 (2011) 248-55.
E. Stanisz, M. Krawczyk, H. Matusiewicz, Solid-phase extraction with multiwalled carbon nanotubes prior to photochemical generation of cadmium coupled to high-resolution continuum source atomic absorption spectrometry, J. Anal. At. Spectrom., 29 (2014) 2388-2397.
D. Shao, C. Chen, X. Wang, Application of polyaniline and multiwalled carbon nanotube magnetic composites for removal of Pb(II), Chem. Eng. J., 185 (2012) 144-150.
Copyright (c) 2019 Analytical Methods in Environmental Chemistry Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
JOURNAL PUBLISHING AGREEMENT
PLEASE PROVIDE US THE FOLLOWING INFORMATION,
Article entitled:
Corresponding author:
To be published in the journal:
Your Status
I am the sole author of the manuscript
- I am an Iranian government employee.
- I am a European government employee
- I am a Asian government
- None of the above
I am one author signing on behalf of all co-authors of the manuscript
- I am an Iranian government employee.
- I am a European government employee
- I am a Asian government
- None of the above
Please tick the above blanks (as appropriate), review the Journal Publishing Agreement, and then sign and date the document in black ink.
Published Journal Article: the author may share a link to the formal publication through the relevant DOI. Additionally theses and dissertations which contain embedded Published Journal Articles as part of the formal submission may be hosted publicly by the awarding institution with a link to the formal publication through the relevant DOI. Any other sharing of Published Journal Articles is by agreement with the publisher only.
Signed: ______________________________________ Name printed: ___________________________________________
Title and Company (if employer representative): _______________________Date: __________________________________